Autophagosome content profiling using proximity biotinylation proteomics coupled to protease digestion in mammalian cells
Summary: invertatop squeeze bottle The ascorbate peroxidase APEX2 is commonly used to study the neighborhood of a protein of interest by proximity-dependent biotinylation.Here, we describe a protocol for sample processing compatible with immunoblotting and mass spectrometry, suitable to specifically map the content of autophagosomes and potentially